ZFIN Image: Lee et al., 2020, Figure 3

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Figure Caption

Figure 3

LEA represses RANKL-mediated NFATc1 expression. (A) Heatmap showing the reads per kilobase per million (RPKM) of c-Fos, NF-κB and NFATc1. Numbers within the figure indicate RPKM values. (B) Total RNA was prepared after treated with M-CSF (30 ng/mL) and RANKL (100 ng/mL) in the presence or absence of LEA (10 μg/mL), and qRT-PCR was performed using primers specific for Nfatc1, c-Fos, and p65. Results represents the means ± SD of three independent experiments. *** p < 0.001 versus only RANKL treatment. (C) Whole cell lysates were prepared from M-CSF/RANKL-treated BMMs with or without LEA (10 μg/mL) for 0, 1, 2, and 3 days, and analyzed by immunoblotting with NFATc1, c-Fos and p65 antibodies. β-Actin was probed as a loading control.

Acknowledgments

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