IMAGE

Fig. 9

ID
ZDB-IMAGE-200306-59
Source
Figures for Lee et al., 2020
Image
Figure Caption

Fig. 9 In vivo AAV9-mediated REEP5 depletion in mice results in increased cardiac fibrosis, activated cardiac ER stress, cardiac dysfunction, and death.

a Immunoblot analysis of AAV9-mediated REEP5 depletion in mice 4 weeks post viral infection. b Histological analyses of AAV9 Scram shRNA and REEP5 shRNA injected mouse hearts at 4 weeks post viral infection. Top panels: H&E, bottom panels: Masson’s trichrome stain. Scale, 20 μm. All images shown are representative of approximately 20 total images captured per condition, n = 3 independent biological replicates. c Transmission electron microscopy analysis of AAV9 REEP5 shRNA-injected myocardium reveals degeneration of muscle fibers and disrupted SR membranes and organization compared to scram controls. M mitochondria, SR sarcoplasmic reticulum, V vacuoles. Scale, 0.5 nm. All images shown are representative of approximately 20 total images captured per condition, n = 3 independent biological replicates. d In vivo echocardiographic heart function assessment of AAV9 scram and AAV9 REEP5 shRNA-injected mice at 4 weeks post viral infection, n = 5. e Echocardiographic M-mode and B-mode measurements showed significantly compromised cardiac function with reduced cardiac ejection fraction and cardiac output, n = 5 independent biological replicates. Asterisks indicate a statistically significant p value in a Tukey’s multiple comparison analysis where ***p < 0.001; data are presented as mean ± SEM. f Immunoblotting analysis of cardiac ER stress markers (GRp78, GRp94, XBP1, ATF4, and CHOP) and ER-dependent apoptosis (caspase 12) upon AAV9-induced REEP5 depletion in vivo in mice, n = 3; p values are shown. g Immunoblots of RTN4, ATL3, and CKAP4 upon AAV9-induced REEP5 depletion in vivo in mice, n = 3 independent biological replicates. Source data containing original uncropped immunoblots are provided as a Source Data file.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.