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Figure 7
4-HPPP-induced changes in endogenous ROS and antioxidants in NSCLC cells. (a) H1299 cells were treated with the indicated concentrations of 4-HPPP for 24 h and 48 h. Afterward, intracellular levels of ROS were measured by the flow cytometry-based DCF-DA assay described in Materials and Methods. (b) Quantitative analysis of (a). (c) Changes in endogenous antioxidants SOD1, SOD2, and PRX1 in H1299 cells following 4-HPPP treatment by Western blotting. (d) Quantitative analysis of (c). ∗
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