IMAGE

Fig. 3

ID
ZDB-IMAGE-200224-9
Genes
Source
Figures for Brock et al., 2019
Image
Figure Caption

Fig. 3

Apoptotic body uptake stimulates Wnt signaling and stem cell division. ac Confocal maximum intensity projections from time-lapse imaging of a p63-positive cell (green), asterisk, engulfing two apoptotic bodies (magenta), arrowheads (scale = 5 µm), see Supplementary Movie  2de Transmission electron micrographs of apoptotic bodies in adjacent basal cells (scale = 2 µm, 500 nm). fh Time-lapse imaging of a dividing p63:EGFP-positive stem cell that has engulfed apoptotic bodies (arrowheads; scale = 5 µm), see Supplementary Movie  3i Quantitation of actively dividing p63:EGFP-positive epithelial stem cells over time after induced apoptosis. jl Confocal images of Wnt-responsive cells (TCF-Siam:GFP positive) after apoptosis. Arrowheads denote increased Wnt activity in a cell engulfing apoptotic bodies (scale = 50 µm). m Mean number of TCF-Siam:GFP-positive cells from individual larvae after stem cell ablation ( n = 5) and treatment with the Wnt inhibitor IWR-1 ( n = 9). Data are from three independent experiments and error bars represent sem; *** p < 0.0001, ** p < 0.0007. One-way analysis of variance (ANOVA) with Dunnett’s mutiple comparisons test (m)

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.