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Figure Caption

Figure 3—figure supplement 3—source data 1.

Verification of myeloid cells on the trunk of the single-HE labeled zebrafish at 7dpf.

(A1–A3) In vivo dual-color imaging of leukocytes (coro1a:GFP) and macrophages (mpeg1:DsRed). (B1–B3) In vivo dual-color imaging of leukocytes (coro1a:GFP) and neutrophils (lyz:DsRed). (C1–C3) In vivo dual-color imaging of neutrophils (lyz:GFP) and macrophages (mpeg1:DsRed). (D) The colocalization percentages of mpeg1+/coro1a+ and lyz+/coro1a+ cells are 59.9% and 26.3%, respectively (n = 3), while lyz+ cells are not colocalized with mpeg+ cells, indicating that the majority of coro1a+ cells (86.2%) on the trunk are lyz+ or mpeg1+ myeloid cells.





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