ZFIN Image: Pálfy et al., 2020, Fig 2

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Figure Caption

Fig 2 Transcription inhibition affects chromatin accessibility only at the miR-430 locus.

a) Correlation dot plot comparing accessibility in α-amanitin-treated embryos with untreated embryos. Significantly (S) and non-significantly (NS) affected peaks (DESeq2 log2 fold change -1.5, 5% FDR), as well as peaks that fall into the miR-430 cluster are indicated. b) Heatmaps of chromatin accessibility in untreated and α-amanitin-treated embryos, grouped based on the expression level of associated genes at sphere stage (expression data taken from [15]; strongly expressed genes are defined as the top 20% genes expressed at sphere stage (RPKM > = 0.419), whereas weakly expressed genes are all other genes (with a minimal expression of RPKM > = 0.004 at sphere stage). c) Genome browser snapshots of untreated and α-amanitin-treated embryos at oblong stage for the hypertranscribed miR-430 gene cluster; id1, a gene with strong zygotic expression; and fbxw4, a gene with weak zygotic expression.

Acknowledgments

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