Figure 1

Figure 1

Influence of environmental parameters (A) temperature and (B) pH on protein activity. Specific lytic activity of the proteins is calculated against S. aureus Sa9. Bars represent the activity (ΔDO₆₀₀ × min⁻¹ × μM⁻¹) of each protein (LysRODI, black; LysA72, gray and CHAPSH3b white). Data are the mean ± standard deviation of three biological replicates. Asterisks indicate statistical differences (p < 0.05; Student’s t-test) between the specific lytic activity when the protein is submitted to the temperature or pH treatment with the activity observed when the protein is tested at 37°C in NaPi buffer, pH = 7.4. The data were expressed as the mean ± standard deviation of three biological replicates.