ZFIN Image: Da Silva-Álvarez et al., 2019, Figure 1

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Figure 1

Pectoral fin amputation induces features of cell senescence. (a) Schematic representation of the fin amputation system used throughout the study. (b) Representative photomicrographs of fins stained for SAbetaGal or phospho‐histone 3 (P‐H3, right panel) after amputations (NA: nonamputated; 8, 16, and 30 dpa: days postamputation). Co‐staining of P‐H3 was done at 8 dpa. Arrowhead shows the amputation plane. (c) Schematic representation showing the different types of samples used in the study (NA: nonamputated; A: amputated; DIS: distal area; PROX: proximal). (d) SAbetaGal activity measured using Galacton substrate after 8, 16, and 30 days postamputation (dpa) (from 5–10 animals per condition). (e) Expression levels by QPCR of cdkn1a (left panel) and cdkn2ab (right panel) genes relative to the housekeeping gene rps11 after 8, 16, and 30 days postamputation (dpa). Results are presented as mean ± SD ***p < .001, **p < .01,*p < .05, n.s. nonsignificant

Acknowledgments

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