Figure 2

Figure 2

Incorporation and proliferation of fresh and cryopreserved spermatogonia. The incorporation and proliferation of fresh (A) and cryopreserved (B,C) vasa::egfp spermatogonia within the testes (A,B) and ovaries (C) of non-sterilized recipients. Testes (arrows) of the control MO-sterilized recipients (D) appear undeveloped under the stereomicroscope (D1), while the histological analysis (D2) displayed a lack of developing germ cells. Recipients of actb:yfp spermatogonia displayed either one (E) or both (F) developed testes (arrows). Developed testes displayed strong green fluorescence originating from donor actb:yfp spermatogonia (F1), while histological analyses displayed clear differentiation of germ cells into spermatozoa (F2). (G) Incorporation of fresh and cryopreserved spermatogonia into non-sterilized (NST) or MO-sterilized (ST) recipients. Values are presented as mean ± SD. Scale bars: (A, B, C) 500 µm; (top panels) 100 µm; (D, E, F) 1 mm; (D1, F1) 200 µm; (D2, F2) 20 µm.