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Figure 2 Cardiac crest-derived cells differentiate into smooth muscle and cardiomyocytes in avian embryos. (A, B) Retrovirally labeled cardiac crest cells (H2B-YFP, green) that migrate into the outflow tract (A, OFT) and pharyngeal arch arteries (B) express smooth muscle actin (SMA, magenta) marker. (C, D) Labeled cardiac crest cells that enter the ventricle express myocardial marker Troponin T (magenta) (C), and myocardial terminal differentiation marker Myosin Heavy Chain (MHC, magenta) (D) enclosed in dashed line. (E, F) Neural crest-derived cardiomyocytes are not actively dividing or undergoing apoptosis, as demonstrated by phosphohistone H3 staining (PH3, magenta) (E) and Caspase 3 staining (magenta) (F). Transverse view of E6 embryos. Separate channels are displayed in supplement 1. Scale bars: 100 μm.