Figure 2

Figure 2

Acute ethanol treatment causes hepatic steatosis and increases macrophage numbers in larval zebrafish. (A,B) Hematoxylin and eosin (H&E) staining of the paraffin sections showing the livers in a control larva (A) and a larva treated with 2% ethanol from 96 to 120 h post fertilization (B). The livers were harvested at 27 h post treatment (hpt). Scale bar, 20 μm. (C,D) Representative images of the whole-mount Oil Red O staining in the control (C) and ethanol-treated larvae (D). Dashed line outlines the liver. Lateral view, anterior is to the top. Oil Red O also stains the swim bladder (asterisk in C) and the residual yolk tissue (asterisk in D). Scale bar, 250 μm. (E,F) Confocal three-dimensional projections showing Tg(mpeg1:YFP)-expressing macrophages (green) in the whole liver at 27 hpt. Phalloidin staining (red) that labels cell cortex is used for recognizing various organs. Ventral views, anterior is to the top. Dashed line outlines the liver. Scale bar, 30 μm. (G) Numbers (mean±s.e.m.) of macrophages per liver at 0 hpt (left) and 27 hpt (right). Statistical significance was calculated by one-way ANOVA and Tukey's post-hoc test. ^**p < 0.01, ns, not significant. This figure is reproduced with permission from Zhang et al. (73) and Disease Models & Mechanisms.