ZFIN Image: Chen et al., 2016, Fig. 1

Image

Figure Caption

Fig. 1

Multicolor Labeling of Superficial Epithelial Cells in skinbow Zebrafish

(A) Schematic drawing of human and zebrafish skin. Zebrafish skin is a simple stratified epithelium without keratinized layers, with a surface covered by transparent mucus. EC, epithelial cell.

(B) Brightfield view of an adult skinbow animal. Green boxes indicate areas where the z stacked confocal images shown in (C) (D) were captured. Scale bars, 2 mm.

(C) Multicolor labeling of skin epithelium in adult skinbow animals from areas shown in (B). Scale bars, 100 μm.

(D and E) Multicolor labeling of corneal and scale epithelium in skinbow. A dissected scale is shown in (E). SECs cover the exposed portion of the scale (top). Scale bars, 100 μm.

Acknowledgments

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Reprinted from Developmental Cell, 36, Chen, C.H., Puliafito, A., Cox, B.D., Primo, L., Fang, Y., Di Talia, S., Poss, K.D., Multicolor Cell Barcoding Technology for Long-Term Surveillance of Epithelial Regeneration in Zebrafish, 668-80, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell