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Fig. 4

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ZDB-IMAGE-190819-17
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Figures for Bercier et al., 2019
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Fig. 4

NMJ function is impaired by severely reduced levels of Dynactin1a. a Sample traces of spontaneous miniature end plate currents (mEPCs) from fast-twitch muscle fibers. b Normalized individual mEPCs from mok m632−/− larvae (blue trace) and their wild-type siblings (black trace). c Average mEPC frequency recorded from wild-type and mutant mok m632−/− larvae. dmEPC amplitude histogram for wild-type and mok m632−/− larvae with Gaussian functions (red traces). e Normalized Gaussian fits from wild-type (black trace) and mok m632−/− larvae (blue trace). f Paired motor neuron skeletal muscle fiber recording each patched and filled with AlexaFluor 488 (calibration bar = 100um). g Motor neuron action potential is evoked by a 2 msec current injection (upper trace) and subsequent muscle EPC is recorded (lower trace). h Average quantal content (evoked EPC amplitude/mEPC amplitude for wild-type and mok m632−/− larvae. Sample traces from paired neuron – muscle whole cell recordings for wild-type and mok m632−/− larvae with stimulus frequencies of i 1 Hz and j 100 Hz. k Magnification of evoked EPCs from mok m632−/− larvae recordings demonstrating postsynaptic EPC failures occurring at 10, 20 and 100 Hz (red asterisks). l Evoked EPC failure rate is significantly higher in mok m632−/− (blue trace) than in wild-type larvae (black trace) for paired recordings 10 Hz, 20 Hz and 100 Hz. Data shown as mean +/−SEM. (c: average = 35.04 ± 9.25, n = 2638 events from 15 fish/ average = 40.86 ± 7.53, n = 4903 events from 24 fish; dn = 2638 events from 15 fish/ n = 4903 events from 24 fish; d: WT peak 1 average = 18.35, WT peak 2 average = 283.19, mutant peak 1 average = 20.42, mutant peak 2 average = 280.00; h: QC = 9.39 ± 3.31, n = 8 pairs/ QC = 10.98 ± 2.59, n = 18 pairs; ln = 8 pairs/ n = 9 pairs)

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurodegener.