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Fig. S5
GRABNE1h can sense endogenous NE release and optic tectal neurons 132 with or without HuC:GRABNE1m overexpression show no difference in spontaneous 133 calcium responses, related to Figure 5. 134 (A,B) Detection of endogenous NE release in the midbrain of GRABNE1h zebrafish 135 evoked by visual looming stimuli. Quantification data is shown in (B). n = 6 fish. 136 (C) Spontaneous calcium activities of optic tectal neurons revealed by jREGCO1a 137 fluorescent signals show no difference with- or without HuC:GRABNE1m expression. 138 Experimental diagram is shown in (C1). Traces for representative calcium responses are 139 shown in (C2). Group data are shown in (C3). n = 7 for transgenic HuC:GRABNE1m 140 zebrafish, and n = 8 for fish without expressing GRABNE1m. 141 142 *p< 0.05 and ***p< 0.001; n.s., not significant (Student’s t-test, Wilcoxon test, or Mann143 Whitney rank sum test).