ZFIN Image: Nowak-Sliwinska et al., 2018, Fig. 22

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Figure Caption

Fig. 22

Imaging of vasculature in the cranial window preparation. Vascular sprouts entering a cranial window tissue isolation chamber. Time sequence of new vasculature (green) migrating toward the top left into a cranial window TIC, past the edge of the PDMS disk (dashed line) (imaged using MPLSM and SHG). Alignment of collagen fibers (white) is evident, and alpha-SMA+ cells can be seen on the PDMS surface (red). The vasculature (green, FITC-dextran) extends by forming perfused loops and sprouts. As the matrix remodels, the vessels also remodel as they advance. a: D1: day 1, D3: day 3, D6: day 6 post-TIC implantation. A pillar structure, which defines the height of the chamber, is indicated with * (from [493])

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