Fig. 12

Fig. 12

Assessing EC autonomous gene function in zebrafish. a–e Whole mount in situ hybridization of 24–48 hpf zebrafish embryos. a–bve-cadherin labeling of the vasculature in the trunk (a) and head (b) of a zebrafish embryo showing vascular-specific labeling. c–dtagln/sm22 (c) and vegfa [308] (d) showing labeling of non-vascular (somitic) tissues in the trunk. e cds2 [309] labeling of both vascular (arrows) and non-vascular tissues. f Confocal micrograph of a growing trunk intersegmental vessel in a 32 hpf Tg(kdrl:mRFP-F)^y286 embryo (red vessels), mosaically expressing Tol2(fli1a:H2B-TagBFP-p2A-egfp-F) transgene (blue EC nuclei, green EC cytoplasm), showing blue, green, and red fluorescent channels and all three merged. g–h Higher-magnification images of GFP fluorescence (g) and merged GFP/BFP/RFP fluorescence (h) in a 48 hpf Tg(kdrl:mRFP-F)^y286 transgenic animals mosaically expressing a Tol2(fli1a:H2B-TagBFP-p2A-egfp-F) transgene in a single EC in a trunk intersegmental vessel. Scale bars = 20 μm (f), 10 μm (g–h). Images in panels f–h are from Ref. [649]