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Fig. 11
Representative flow-chart of flow cytometry and cell sorting experiments. Overview of samples collection (tumors, organs bearing metastasis, and blood) from mice and the procedure to obtain a single-cell suspension after tissue dissociation, erythrocytes lysis, and cell labeling with fluorescent or paramagnetic-coupled antibodies. Analysis of blood-circulating cells only requires a red blood cell lysis prior to incubation with antibodies of interest. Intracellular antigens can be detected by adding a cell permeabilization step. Fluorescent labeled cells are analyzed by flow cytometry and sorted by FACS while cells labeled with paramagnetic-coupled antibodies can be sorted by MACS. MACS can be used to pre-enrich cells for subsequent FACS sorting. Cell populations of interest are identified in by dot plots analysis of collected data