Fig. 5-S3

(A) Dorsal views of wt and MZfz7a/b mutant embryos, as well as MZfz7a/bmutant embryos expressing Opto-Fz7 and injected with 9-cis-retinal at the end of gastrulation (bud stage, 10 hpf) labeled by in situ hybridization for hgg (ppl), ntl (nc), and papc (paraxial mesoderm). Embryos were exposed to light. Scale bar, 250 μm. (B–D) Notochord length (B) and width (C), and width of the paraxial mesoderm (D) in wt MZfz7a/b mutant embryos, as well as MZfz7a/b mutant embryos expressing Opto-Fz7 and injected with 9-cis-retinal, compared to uninjected mutant and wt embryos from Figure 1—figure supplement 1B-D. N = 78 (wt), 63 (MZfz7a/b), 21 (MZfz7a/b plus Opto-Fz7), *p<0.05, ***p<0.001 (ANOVA followed by Tukey’s multiple comparison test for B and D, Kruskal-Wallis test followed by Dunn’s multiple comparison test for C)