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Fig. 5
Partial antisense repression of Cripto-1 rescues inhibition of regeneration by BDP. a) Translation-blocking cripto-1 morpholino oligo (MO) transiently knocked down Cripto-1. The morphants developed the characteristic one-eyed pinhead phenotype by 2 days post-fertilization (dpf). b) The control and cripto-1morphants were exposed to DMSO or beclomethasone dipropionate (BDP) at 2 dpf following amputation. The dotted lines mark the plane of amputation. Regenerative progression was evaluated and pictures were taken at 3 days post-amputation (dpa). In each replicated experiment approximately 80% of the cripto-1morphants were resistant to inhibition of regeneration by BDP exposure.