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Fig. 2
Activation of the regulatory region of PoACTC1 in zebrafish embryos and olive flounder. The fluorescent protein reporter of the PoACTC1 regulatory region (pPoACTC1-P2877) was digested with the restriction endonuclease SalI, then the DNA solution was injected into one- or two-cell-stage embryos using an air pressure microinjector. Digital images of embryos were captured using an Olympus macro zoom fluorescence microscope. (A) Zebrafish embryos at 1 and 2 dpf; scale bar: 200 μm. (B) Olive flounder at 6 dpf; scale bar: 500 μm.
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