ZFIN Image: Colucci-Guyon et al., 2019, Fig. 9

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Figure Caption

Fig. 9

HAB selectively labels live human primary neutrophils over other blood cell types. (a–c and d–f) confocal fluorescence and DIC imaging of HAB (10 μM) in live human neutrophils following excitation at 448 nm and detection in the 550–650 nm range. Single 0.4 μm optical sections are shown. (a–c) show resting, non-activated, non-adherent neutrophils. (d–f) show activated, adherent neutrophils: note HAB staining of neutrophils granules (g–i): confocal fluorescence and DIC imaging of HAB (10 μM) in live human lymphocytes (lc), erythrocytes (ec), monocytes (mc) and thrombocytes (tc) using the same settings as in (a–c) and (d–f). Single 0.4 μm optical sections are shown. The yellow-orange color is indicative of the fluorescence seen with the naked eye. Asterisk = nucleus.

Acknowledgments

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