ZFIN Image: Colucci-Guyon et al., 2019, Fig. 6

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Figure Caption

Fig. 6

HAB labels zebrafish neutrophil granules. (a–l) Confocal fluorescence imaging of HAB (10 μM) in live transgenic 72 hpf zebrafish larvae following excitation at 448 nm under equilibrium conditions. Detection parameters were as follows: for HAB/mCherry: λ_ex 448 nm, λ_em 550–650 nm, mCherry: λ_ex 552 nm, λ_em 660–750 nm using sequential modes of acquisition. For GFP/HAB: GFP λ_ex448 nm, λ_em 500–520 nm, HAB: λ_ex 448 nm, λ_em 550–650 nm using sequential modes of acquisition. Maximum intensity Z-projection images (2 μm serial optical sections) are shown. (m–p) High-resolution DIC and confocal fluorescence imaging of HAB (10 μM) in live 72 hpf zebrafish larvae harbouring red neutrophils. Arrows point to HAB-labeled granules according to the DIC images in neutrophils. A single 0.4 μm optical section is shown. Boxes in (c), (g) and (k) indicate the regions magnified in the insets (d), (h) and (l) respectively. Abbreviations used: A (aorta); N (notochord); V (vein); asterisk = nucleus. See ESI for Videos S5 and S6† related to (m–p).

Acknowledgments

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