ZFIN Image: Lin et al., 2016, Figure 2

Image

Figure Caption

Figure 2

Effect of environmental Ca²⁺ concentrations on whole-body 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) content in 3 day post-fertilization (dpf) zebrafish embryos. One to two cell-stage zebrafish embryos were incubated in low- (0.02 mM Ca²⁺) and high-Ca²⁺ (2.0 mM Ca²⁺) water, respectively, for three days. The low-Ca²⁺ (LCa) group showed a higher level of 1,25(OH)₂D₃ than the high-Ca²⁺ (HCa) group. Whole-body 1,25(OH)₂D₃ content was measured with an ELISA kit (E0467GE, EIAlab, Wuhan, China). Values are the mean ± SEM (n = 5). Student’s t-test, ** p < 0.01.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.