ZFIN Image: Stankiewicz et al., 2019, Fig. 3

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Figure Caption

Fig. 3

Daily proliferative activity in the brain of normal 1-year-old zebrafish. Cell proliferation in the cerebellum over a 1 to 3 d BrdU/EdU pulse-chase. A, Schematics of representative cell division modes and cell color on the day of BrdU pulse (day 0) and EdU chase (day 1). RV, Rhombencephalic ventricle. B, Representative image of labeled cells in the cerebellar niche and outside parenchyma on day 3 of BrdU/EdU pulse-chase, and corresponding schematics. Arrows indicate posterior mesencephalic layer (PML). Scale bar, 50 μm. C, Number of labeled cells in the cerebellar neurogenic niche. D, Number of labeled cells in the whole cerebellum. E, Percentage of labeled cells in the neurogenic niche versus whole cerebellum. Cell counts conducted in brain sections. Red represents BrdU-only. Green represents EdU-only. Yellow represents BrdU/EdU colocalized. N = 5 or 6 fish per time point. Data are mean ± SEM. *p < 0.05, relative to day 1.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.