ZFIN Image: Alkobtawi et al., 2018, Fig. 2

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Figure Caption

Fig. 2

Generation of Pax3-GFP transient transgenic embryos.(A) pax3 or CMV promoters fused to GFP were injected into Xenopus embryos as described to generate transient transgenic embryos. (B) GFP fluorescence was examined at neurula stage (stage 19). (C) CMV-GFP transient transgenic embryo, showing ubiquitous expression. (D) Pax3-GFP transient transgenic embryo showing expression restricted to the neural folds. (E, F) In situ hybridization (ISH) for comparison of endogenous Pax3 expression with GFP expression in Pax3-GFP transient transgenic embryos. (E) ISH for pax3 at the indicated stages. (F) ISH for GFP in Pax3-GFP embryos at the indicated stages. Note the similar expression of Pax3 and GFP. 92% of Pax3-GFP embryos exhibited expression in the neural folds (n= 210).

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Reprinted from Developmental Biology, 444 Suppl 1, Alkobtawi, M., Ray, H., Barriga, E.H., Moreno, M., Kerney, R., Monsoro-Burq, A.H., Saint-Jeannet, J.P., Mayor, R., Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development, S202-S208, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.