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Fig. 2
Tmie-GFP is present in the hair bundles of MET mutants.
Confocal images of the bundle region in hair cells of the inner ear lateral cristae in 6 dpf larvae. Larvae expressing transgenic Tmie-GFP in the genetic backgrounds of wild type (A), and homozygous mutants for the tip link protein Pcdh15a (B, pcdh15apsi7), the accessory protein Lhfpl5a (C, lhfpl5atm290d), and the Golgi-localized protein Tomt (D, tomttk256c). Tomt-deficient fish lack Tmc expression in hair cell bundles [11], presumably mimicking the condition of a triple Tmc knockout. Arrowheads indicate splayed hair bundles. n = 8 each genotype. Scale bar is 5μm.
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