ZFIN Image: Cau et al., 2019, Fig. 3

Image

Figure Caption

Fig. 3

Impairing Notch activity modifies the timing of PhR differentiation and favors early PhR fate at the expense of late PhR fate.

(A-B) Representative confocal sections showing the expression of the Tg(aanat2:gfp)^y8(aanat2:gfp) transgene in Tg(hsp70l:dnXla.Rbpj-MYC)^vu21 (referred to as Notch KD) and wt siblings at 42 hpf. (C-F) Expression of exorh and PT in Tg(hsp70l:dnXla.Rbpj-MYC)^vu21 and siblings at 48 hpf (C-D) and 66 hpf (E-F). (G) Counts of Tg(aanat2:gfp)^y8+ cells at various stages in Tg(hsp70l:dnXla.Rbpj-MYC)^vu21 and siblings. The stage is indicated on the x-axis. (H) Counts of exorh+ and PT+ cells in Tg(hsp70l:dnXla.Rbpj-MYC)^vu21 and sibling embryos after in situ hybridization. Stages are 48 hpf for exorh+ and 66 hpf for PT+ cells. Scale bars represents 10 μm. Heat shock was performed at 14 hpf. Error bars represent SD. *p < 0.05, **p < 0.001 using a Mann Whitney test. Underlying data can be found in S1 Data. exorh, exorhodopsin; hpf, hours post fertilization; KD, knock-down; PhR, photoreceptor; PT, parietopsin; wt, wild type.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.