ZFIN Image: Shi et al., 2018, Fig. 8

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Figure Caption

Fig. 8

DO-SRS microscopy, in combination with fluorescent labeling, tracks lineage-specific metabolism during zebrafish embryogenesis. a–c SRS microscopic signal and the colocalization with fluorescence from Tg(kdrl::EGFP) reporter in zebrafish embryos that were incubated in egg solution containing 20% D₂O from 0 to 24 hpf, from 24 to 34 hpf, or from 34 to 48 hpf. Dashed curves outline the yolk sac extension (labeled as Y) and the GFP-positive hemangioblast (labeled as H) that has strong CD_P signal. Arrows point to the GFP-positive cells that are likely differentiating angioblasts in b. d, e Intensity profiles that quantify the CD_P signal within the yellow rectangle for 0–24 and 34–48 hpf probing, respectively. x axis shows the position along the length of the box from top to bottom, and y axis shows the average intensity across the width of the box. Scale bar = 20 μm

Acknowledgments

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