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Fig. S3
Metronidazole treatment does not cause ONL or RPE apoptosis in nontransgenic larvae.
(A-D) Transverse cryosections stained for TUNEL (red). No TUNEL+ cells were detected in nontransgenic larvae (A,C) treated with and without MTZ. (E,F) Quantification of TUNEL+cells/section in the ONL (E) and RPE (F). While ONL death appeared to be elevated in unablated rpe65a:nfsB-eGFP+ larvae, the increase was not significant (p = 0.1848), and no significant elevation of TUNEL+ cells was detected in the RPE (Mann-Whitney U test, *P<0.05, **p<0.005, ***p<0.0005). Scale bar = 40μm.
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