ZFIN Image: Lalonde et al., 2018, Fig. 5

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Figure Caption

Fig. 5

The “m-Inta11” and “2PΔEpi” enhancer elements display overlapping domains of activity in the zebrafish pectoral fin fold mesenchyme at 72hpf. (A-D) Zebrafish pectoral fin at 72hpf of Tg(2PΔEpi:mCherry) x Tg(m-Inta11:eGFP) double transgenic fish. Both regulatory elements drive overlapping reporter expression in posterior fin fold mesenchyme (yellow arrow) (B-D), providing evidence they may both be co-activated by Hoxa13a and Hoxd13a proteins. Note the absence of reporter activity in anterior fin fold mesenchyme (B-D). Due to inconsistencies within transgenic lines, individual cells contain variable amounts of eGFP and mCherry protein and accounts for the colour variation observed in different mesenchymal cells (D). Brightfield (A), Fluorescent (B, C), and merged (D) images are displayed. ED, endoskeletal disc; FF, fin fold. Scale bar, 3 mm.

Acknowledgments

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