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Fig. 4 Knockdown of sinup expression with sinup-specific morpholino (sinup MO) alters cell movement along CE. sinup MO was injected in one- to two-cell stage embryos. Control embryos and the injected embryos were fixed at 90% epiboly (A,E), tail bud (B,F,I,K,L,M,O,P), and five-somite stages (C,D,G,H,J,N). The embryos were subjected to whole-mount in situ hybridiation with myoD (I,M), pax2.1 and myoD (J,N), dlx3 (K,O), and DAPI staining (L,I). (A–D) and (I–L) are wild type; (E–H) and (M–P) are sinup MO-injected embryos. Lateral view: A–C,E–G, L–P; dorsal view: D,H,I–K, M–O.
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