ZFIN Image: Earley et al., 2018, Fig. 2

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Figure Caption

Fig. 2

Significant Reduction of Intestinal Macrophages but No Change in Neutrophil Number in irf8^−/− Mutants

(A) Representative maximum intensity projections of confocal z stacks of the adult S7 intestinal region. Double transgenic irf8 mutants and siblings carrying the macrophage reporter mpeg1:GFP (green) and neutrophil reporter lyz:mCherry (red) were analyzed.

(B) Schematic of the S1–S7 segmentation of the adult zebrafish intestine. The green square inside of S7 represents the approximate region where images in (A) were taken.

(C) Quantification of the number of macrophages (mpeg1+) from a representation of all intestinal segments S1–S7. ^∗p < 0.05.

(D) Quantification of the number of neutrophils (lyz+) from a representation of all intestinal segments S1–S7.

(E) qPCR analysis of neutrophil marker mpx expression shows no significant difference between irf8 mutants and siblings.

3 or more independent animals were used per mutant and sibling groups for all quantifications. Error bars show SEM. Student’s t test was used to determine statistical significance. SIB, wild-type and heterozygous siblings.

Acknowledgments

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