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Fig. 2
BMP redirects fate of mouse NMPs from paraxial to lateral mesoderm.
(A) Heterotopic grafting from ubiquitous GFP embryos (Gilchrist et al., 2003) of NMP region fated for paraxial mesoderm at 2–5 somite stage (E8.0) into the posterior primitive streak region fated for lateral and ventral mesoderm, followed by 48 hr culture. (i) Posterior view (ii) Lateral view (iii) Representative embryo immediately after grafting showing position of GFP +grafted cells (iv) Representative embryo after 48 culture showing that descendants of grafted cells have adopted a lateral fate (arrowheads). (B) qPCR at indicated time points during the differentiation of EpiSCs into NMPs then treated with FGF2 or FGF2 +BMP4. Data shown relative to the housekeeping gene TBP. (C) Immunofluorescence detection of indicated markers in in vitro derived NMPs and their differentiating derivatives. (D) Flow cytometry of Flk1-GFP in the differentiating derivatives of in-vitro derived NMPs. (E) Immunofluorescence detection of indicated markers in the differentiating derivatives of in-vitro derived NMPs.