ZFIN ID: ZDB-PUB-180609-11
BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
Row, R.H., Pegg, A., Kinney, B., Farr, G.H., Maves, L., Lowell, S., Wilson, V., Martin, B.L.
Date: 2018
Source: eLIFE   7: (Journal)
Registered Authors: Farr III, G. Hank, Martin, Benjamin, Maves, Lisa, Row, Richard H.
Keywords: developmental biology, mouse, stem cells, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Body Patterning/genetics
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism*
  • Cell Differentiation/genetics
  • Cells, Cultured
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism*
  • Gene Expression Regulation, Developmental
  • Mesoderm/cytology
  • Mesoderm/embryology
  • Mesoderm/metabolism*
  • Mice
  • Signal Transduction*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 29877796 Full text @ Elife
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ABSTRACT
The mesodermal germ layer is patterned into mediolateral subtypes by signaling factors including BMP and FGF. How these pathways are integrated to induce specific mediolateral cell fates is not well understood. We used mesoderm derived from post-gastrulation neuromesodermal progenitors (NMPs), which undergo a binary mediolateral patterning decision, as a simplified model to understand how FGF acts together with BMP to impart mediolateral fate. Using zebrafish and mouse NMPs, we identify an evolutionarily conserved mechanism of BMP and FGF mediated mediolateral mesodermal patterning that occurs through modulation of basic helix-loop-helix (bHLH) transcription factor activity. BMP imparts lateral fate through induction of Id helix loop helix (HLH) proteins, which antagonize bHLH transcription factors, induced by FGF signaling, that specify medial fate. We extend our analysis of zebrafish development to show that bHLH activity is responsible for the mediolateral patterning of the entire mesodermal germ layer.
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