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Fig. S1
DAPI staining of the retina in PGUSG larvae.
(a) One optical section of confocal image of the retina with DAPI staining in a 3-dpf PGUSG larva. The nasal is upwards, the dorsal is leftwards. (b,c) Zoom-in and color separate view of the boxed region in (a). We chose three sections of each retina to count the number of Sypb-EGFP positive RGCs and DAPI-stained RGCs. Taking DAPI-stained RGCs as total RGCs, we found that 14.5 ± 0.5% (mean ± s.e.m.) of the RGCs were EGFP-positive in PGUSG at 3 dpf. As ~ 50% of RGCs could be labelled by the pou4f3 driver25, our PGUSG lines can at most label ~ 30% of pou4f3-positive RGCs. The calculation was based on data obtained from 3 larvae. Scale bar, 5 μm.