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Fig. 5
Adverse effects of BPA treatment on ovaries. (A) The morphology of ovaries of F0 females during the treatment was monitored by fluorescence observations performed at 1-month intervals (F0). Photographs of representative F1 females from the control (Control) and BPA-treated (BPA) groups were taken before histological analysis (F1). Photographs of whole fish and ovarian tissues under bright field (B. field) and GFP filter views (GFP) are shown. (B) Sections of control-treated (Control) and BPA-treated (BPA) females. Developmental stages of oocytes are indicated according to Selman et al. as follows: I (early vitellogenic oocytes, perinucleolar oocyte), II (Mid-vitellogenic oocytes) and III (fully grown oocytes) (Selman et al., 1993). The scale bars indicate 50 μm. (C) Number of oocytes in stages I, II or III in sections of control-treated (Control) and BPA-treated (BPA) females were counted in three sections prepared from three different fishes. Vertical lines indicate standard deviation. ** indicate statistically significant differences between the values for the BPA-treated group and the control group at the P < 0.01 level.