ZFIN Image: Rosowski et al., 2018, Fig. S4

Image

Figure Caption

Fig. S4

Imaging of phagocyte clusters.

A. Individual channels of images from Fig 4C are shown. Representative z projection images of dual macrophage-nuclear (mpeg1:mcherry-H2B) and neutrophil (lyz:BFP) labeled larvae infected with YFP- or GFP-expressing A. fumigatus TBK1.1 (Af293) or TFYL49.1 (CEA10) strains and imaged days 1 and 2 post injection. Scale bar represents 20 μm, inset scale bar represents 5 μm. Examples of spore germination inside the cluster are marked in insets with arrowheads. B. Nuclear macrophage labeled larvae (mpeg1:mcherry-H2B) were infected with GFP-expressing TFYL49.1 (CEA10). Z-projection (mcherry, GFP) or single slice (BF) images of the same larvae on days 2, 4, and 5 dpi are shown. Extrusion at 4 dpi is marked with an arrow. Scale bar represents 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.