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Fig. S5
Comparing fluorescent images of GFP-collagen I transgenic fish versus Second Harmonic Generation (SHG) imaging.
(A) A maximum projection confocal image and (B) single z-plane image of a 10 dpf GFP-collagen I transgenic fish directly compared to a single z-plane signal from a second harmonic generation (SHG) microscopy image of the same fish (C). (D) A single zplane SHG microscopy image of a non-transgenic zebrafish. M, muscle fibres; O, orthogonal collagen fibrils. Scale bar = 15 μm.
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Reprinted from Developmental Biology, 441(1), Morris, J.L., Cross, S.J., Lu, Y., Kadler, K.E., Lu, Y., Dallas, S.L., Martin, P., Live imaging of collagen deposition during skin development and repair in a collagen I - GFP fusion transgenic zebrafish line, 4-11, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.