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Fig. 7
The testis dissected from a Tg(gsdf:GFP) transgenic line was stained with propidium iodide and cleared with the PACT protocol. Imaging and 3D reconstruction of the whole testis were performed as shown in Supplementary Fig. S5 and Supplementary Videos S6 and S7. (a) A 2D optical section at 237 μm in depth. Nuclei of undifferentiated spermatogonia are identified through the testis by their larger volume and low fluorescence intensity (arrows). (b) Examples of 3D surface reconstructions of germinal niches containing clustered undifferentiated spermatogonia. (c) High magnification view of two nearby niches located in adjacent seminiferous tubules and displaying six nuclei each. (d) High magnification view of a niche displaying two nuclei of undifferentiated spermatogonia. Nuclei are in grayscale or magenta. Surface reconstructed Sertoli cells and nuclei of undifferentiated spermatogonia are in green and magenta, respectively. Scale bars: 50 μm (a,b) and 20 μm (c).