|
Fig. S15
Procedure for selecting a confocal slice of the most apical lens epithelium
(A) 3D confocal image of Tg(h2afva:GFP; EF1α:mCherry-CAAX) transgenic fish lens.
(B) Schematic picture of zebrafish lens epithelium and z-axis slice level. Eight slices containing the anterior lens epithelium from the anterior to posterior direction are shown. The interval between neighboring slices is 1 μm. Three posterior slices, #6–8, contains the lens fiber region. Thus, the #5 slice is the most suitable for analysis of cell intercalation and epithelial rearrangement, because CAAX-labeled plasma-membranes correspond to the adherens junction complex-containing domain.
(C) The confocal slice containing the most apical lens epithelium just adjacent to the lens fiber core (indicated as the #5 slice in B), which was used to make a movie and to analyze cell division, cell intercalation, and area expansion.