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Fig. 5
Angiotropism in zebrafish xenograft of cutaneous melanoma. (A) A larva injected with C8161-GFP cells, displaying an angiotropic cell (in the square) extending along the external surface of the caudal vein. (B–D) are time-lapse images of the same angiotropic cell taken at 0, 4 and 8 hours after the beginning of the imaging. The images were obtained employing a Zeiss LSM 700 confocal microscope (25 × oil objective), starting from 30 hours post injection. Scale bar is 20 µm, green shows cutaneous melanoma cells, white asterisk shows intravascular melanoma cells, red shows zebrafish blood vessels.
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