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Fig. S3
Deletion screen of kcnk5b reveals essential role of C-terminus.
Using specific guide RNAs against the last exon of knck5b encoding the last transmembrane and cytoplasmic tail of the channel, we screened injected founders for evidence of overgrowth. We identified localized clones having specific overgrowth of the fins. B) Analysis of the changes in the overgrown tissues demonstrated presence of local deletions in kcnk5b predicted to cause truncation of the channel in a comparable location as to the alf mutation (C).
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