Fig. 3

Orcein stains for elastin in plates of connective tissue in spotted gar and zebrafish. Sagittal sections of caudal fin regions of (A) a 24 mm TL spotted gar larva (∼22 dpf) and (B) a 13 mm TL zebrafish (21 dpf) stained with Orcein (red‐rusty color) and counter stained with Gills hematoxylin (blue‐purple). Scale bar = 100 μm. White elongated triangles indicate hypural 1 and black arrows point at the hypural diastema. hd, hypural diastema; hyp, hypural; nc, notochord; oc, opisthural cartilage; pcta, anterior plate of connective tissue; pctp, posterior plate of connective tissue; phy, parhypural; rbc, red blood cells; vas, vasculature.