|
Fig. S9
bsx mutant larvae exhibit no increase in apoptosis in the pineal complex.
(A-D) Tg(foxD3:GFP) embryos at (A,B) 30 hpf or (C,D) 72 hpf fixed and stained for apoptosis by TUNEL staining. Dorsal views, anterior to the left. Scale bar: 100 µm. Images show superimposition of widefield epifluorescence for detection of GFP (maximum intensity projections of all z-planes encompassing the pineal complex), and transmitted light DIC images for chromogenic TUNEL stain (minimum intensity projection of all z-planes encompassing the pineal complex). Apoptosis in the pineal complex is low in both wildtype and bsx mutant embryos (n≥2 for all stages and genotypes).