ZFIN ID: ZDB-IMAGE-180723-31
Figures for Enya et al., 2018

Figure Caption/Comments:

Fig. 7

Overexpression of cyp7a1 in the liver restores the amount of total BA in tumor-bearing larvae. (A) The structure of fabp10a:mCherry-P2A-cyp7a1. The white triangles represent the recognition sequence by I-SceI meganucleases. (B,C) Representative images of mCherry-P2A-cyp7a1 transgene expression in the liver. Control refers to Tg(pInt-Gal4)+/Tg; Tg(UAS:EGFP)+/Tg; Tg(fabp10a:mCherry-P2A-cyp7a1)+/Tg, and tumor-bearing larvae to Tg(pInt-Gal4)+/Tg; Tg(5×UAS:EGFP-P2A-krasG12D)+/Tg; Tg(fabp10a:mCherry-P2A-cyp7a1)+/Tg. Scale bar: 500 µm. Bright-field (B) and mCherry (C) images are shown. (D) qPCR analysis for detecting mCherry-P2A-cyp7a1 mRNA in the liver and the rest of the body at 7 dpf. The scores are normalized to expression of rpl13a. The data harbors 3 biological replicates, each containing 3 larvae. Error bars represent means±s.e.m. CypOE – and + indicate the absence and presence of Tg(fabp10a:mCherry-P2A-cyp7a1), respectively. (E,F) Measurement for systemic BA levels at 7 (n=10 per a group) and 9 (n=30-31 per a group) dpf. The scores are relative index determined using bile acids as standards (see Materials and Methods). Statistical significance was tested using Student's t-test (unpaired, one-tailed). Data are representative of at least two independent experiments.

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.