|ZFIN ID: ZDB-IMAGE-180723-31|
Overexpression of cyp7a1 in the liver restores the amount of total BA in tumor-bearing larvae. (A) The structure of fabp10a:mCherry-P2A-cyp7a1. The white triangles represent the recognition sequence by I-SceI meganucleases. (B,C) Representative images of mCherry-P2A-cyp7a1 transgene expression in the liver. Control refers to Tg(pInt-Gal4)+/Tg; Tg(UAS:EGFP)+/Tg; Tg(fabp10a:mCherry-P2A-cyp7a1)+/Tg, and tumor-bearing larvae to Tg(pInt-Gal4)+/Tg; Tg(5×UAS:EGFP-P2A-krasG12D)+/Tg; Tg(fabp10a:mCherry-P2A-cyp7a1)+/Tg. Scale bar: 500 µm. Bright-field (B) and mCherry (C) images are shown. (D) qPCR analysis for detecting mCherry-P2A-cyp7a1 mRNA in the liver and the rest of the body at 7 dpf. The scores are normalized to expression of rpl13a. The data harbors 3 biological replicates, each containing 3 larvae. Error bars represent means±s.e.m. CypOE – and + indicate the absence and presence of Tg(fabp10a:mCherry-P2A-cyp7a1), respectively. (E,F) Measurement for systemic BA levels at 7 (n=10 per a group) and 9 (n=30-31 per a group) dpf. The scores are relative index determined using bile acids as standards (see Materials and Methods). Statistical significance was tested using Student's t-test (unpaired, one-tailed). Data are representative of at least two independent experiments.
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