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Fig. 1

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ZDB-IMAGE-180711-61
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Figures for Frøyset et al., 2018
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Fig. 1

Generation of zebrafish with astroglial over-expression of DJ-1. A, Transgenic zebrafish expressing Flag-tagged DJ-1, Tg(gfap:egfp-2A-flag-zDJ-1), were generated using ISce1-transgenesis. Flag-tagged DJ-1 expression in astrocytes was controlled by Glial Fibrillary Acidic Protein (GFAP) promotor elements. A viral 2A peptide allows expression of GFP and Flag-DJ1 as uncoupled proteins from the same transgene. B, Western blot shows expression of Flag-DJ1 from total protein extracts of larvae 24–120 hpf. Ponceau S staining is used as loading control. C, GFP expression localized astroglia, neural retina and spinal cord was observed from 48 hpf. c1–3, Immunoblots of brain sections (4 dpf) showing astroglial GFP and neuronal tyrosine hydroxylase expression. Tyrosine hydroxylase was used as a marker for dopaminergic cells.

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