ZFIN Image: Zhang et al., 2018, Fig. S6

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Figure Caption

Fig. S6

Dopaminergic and cholinergic efferent neurons innervate lateral-line neuromasts. (a-d) Neuromast immunostaining of ChAT (cyan, a) and TH (yellow, c) label cholinergic and dopaminergic efferents respectively. Colocalization of Vamp2 (magenta, b), a neuronal presynaptic marker, with ChAT and TH (d) demonstrates that both types of efferents make presynaptic contacts on or near neuromasts. (e-h) Neuromast afferents expressing neurod:GFP (cyan, e) demonstrate that cumulatively, Acetylated tubulin (yellow, g) and Vamp2 (magenta, f) immunostaining can be used to label afferent and efferent innervation respectively. Scale bar = 5 μm.

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