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Fig. 4
Maternally loaded oep mRNA is globally degraded, at least partially due to the abundance of destabilizing codons. (A) Experimental setup. (B) Embryos treated with flavopiridol lack oep expression at 60% epiboly. The number of embryos with the indicated expression pattern among the total number examined in three biological replicates is shown. All side views are shown with dorsal towards the right. Scale bar: 250 µm. (C) RT-qPCR analysis of nascent oep transcript in control versus flavopiridol-treated embryos. Data are normalized to eif4g2a. The experiment was performed in biological triplicate. (D) oep coding sequence is highly enriched for destabilizing codons. The codon stability score of oep is lower than that of highly stable (ik and hsp90) and even highly unstable (trmt12 and hps4) mRNAs as identified by Bazzini et al. (2016). The distribution of stabilizing (green) and destabilizing (red) codons along the coding sequence is shown (top), along with the same data sorted by codon stability score (bottom). The number of codons in the coding sequence is indicated in parentheses for each gene. See the supplementary Materials and Methods for further details.