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Fig. S3
Absence of caudal fin defects in Tg(Inta11:NTR)larvae at 30dpf following metronidazole treatment, small percentage of larvae display major unrelated caudal fin defects in all treatment groups. Developmental delays in Tg(Inta11:NTR)larvae following metronidazole treatment.
(A-C) Whole-mount view of larvae used for Fig 8G–8L. (D-F) Example of major caudal defects present in all treatment groups. (G-I) Comparison of larvae development between Inta11: NTR—MTZ and Inta11: NTR + MTZ. At 30 dpf, Inta11: NTR + MTZ larvae do not show caudal fin ray defects (black arrow) (A) compared to control larvae (black arrows) (B-C). Calcification of entire spinal cord and first 3–4 ribs (red arrow) used for stage matching between treatment groups. Standard length present in bottom right corner (A-C). Pectoral fins were dissected and imaged for Fig 8G–8L. All treatment groups (WT + MTZ, Inta11: NTR—MTZ, Inta11: NTR + MTZ) have a small percentage of larvae with major unrelated caudal fin defects (Yellow arrow) (D-F). Number of larvae with phenotype present in bottom right corner of each panel (D-F). Inta11: NTR + MTZ larvae show >5 day developmental delay at 20dpf (I). Inta11: NTR—MTZ larvae at 15dpf (G), and 20dpf (H) shown as comparison. Beginning of caudal fin ray formation detected in 15dpf Inta11: NTR—MTZ (G), and 20dpf Inta11: NTR + MTZ larvae (I) (Green arrow). All caudal fin rays present at 20dpf in Inta11: NTR—MTZ larvae (blue arrow) (H). Scale bars: 50μm in A-C, D-F, H; 30μm in G, I.