IMAGE

Fig. 4

ID
ZDB-IMAGE-180501-4
Source
Figures for Gross-Thebing et al., 2017
Image
Figure Caption

Fig. 4

Dnd Is Required for Maintenance of Germline RNA Expression and for Inhibition of Somatic RNA Expression

(A) Upregulation of the muscle-specific master regulator myoD and downregulation of the germline-specific marker vasa within Dnd-deficient PGCs, as determined by using RNAscope in situ hybridization. Confocal plane images (4.1 μm thick) were acquired at 24 hpf within myoD-expressing muscle precursors. Directional cell migration was inhibited by knockdown of Cxcl12a. Scale bars, 20 μm.

(B) Graph presenting the number of vasa- and myoD-positive RNA spots detected within PGCs in control embryos and embryos knocked down for Dnd (quantification of results as presented in A). p Values determined using Mann-Whitney U test with α = 0.05. Data are presented as median ± IQR.

(C) PGCs (red) captured in transgenic embryos expressing a muscle-specific transgene unc45b:tfp (blue) labeling muscle precursors in control and Dnd-deficient cells. Directional cell migration was inhibited by knockdown of Cxcl12a. Confocal plane images, 5 μm thick, captured at 24 hpf. Scale bars, 20 μm.

(D) Graphs presenting signal intensity ratios of the muscle-specific transgene (as in C). Signal intensities within the PGC (1) were compared to intensities in a neighboring area of the same shape (2). p Values determined using Student’s t-test with α = 0.05. Data are presented as median ± IQR.

n, number of cells; F′, farnesylated; KD, knockdown; hpf, hours post fertilization.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 43, Gross-Thebing, T., Yigit, S., Pfeiffer, J., Reichman-Fried, M., Bandemer, J., Ruckert, C., Rathmer, C., Goudarzi, M., Stehling, M., Tarbashevich, K., Seggewiss, J., Raz, E., The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation, 704-715.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell